Speakers - 2026

Title: Quality assessment of urease base organic bio fertilizer using ureolytic bacteria by laboratory scaling in readiness for pilot scaling phase II

Abstract

Purpose: The rapidly increasing importance of enzyme urease applications has drawn attention to the need of enzyme study from tannery  sludge   or  vertebrate   excreter    from   cow   urine . It prompted the present study to hunt a promising bacterial strain with the desired nature  for   urease biofertilizer production  along side  with  agro-waste  bio stimulant . Microbial urease can exist in two possible states  either  in  tannery  sludge   or  vertebrate   excreter    from   cow     urine .   To  this   effect, it   might  have had  been either through  intracellularly and directly associated with ureolytic microorganisms, or extracellularly, after being released from cells. Urease-producing bacteria are of particular interest for the production of complex bio-enzymes and are known to produce aftermath   of  utilization from  tannery  sludge through fermentation.  Furtherance’s  the processes once  again  an appreciable amount  of  the  isolates  of  uroelytic  bacteria from   tannery  waste  sludge or  vertebrate   excreter    from   cow   urine   ; which   might   have  had played  a significant role in the growth of plants, animals/ microorganism in their unique endemic consortium within a given habitat.  

Methods: Further investigations were painstakingly  made on the quality of top surface soils for both control farm land and contaminated land fill; thus, they are found to be quiet significantly different in their soil texture, permeability, color etc., respectively. Isolation of bacteria isolates, Characterization, and biochemical identification from tannery sludge was carried out in due course.

Results: The bacteria isolate that were significantly and namely identified are as follows; (Proteus vulgaricus, Klebsiella species, Pseudomonas aeruginosa, Bacillus sp) etc., respectively. All the isolates were found to be Gram +ve,, motile and Klebsiella sp were found to be capsule staining, and urease activities, catalase test, oxidase test were all positives. Effect of metal ion concentration of ureolytic strain of bacteria were duly accentuated as well, the results revealed HgCl and CrSO4concetration at 2% most organisms’ growth were –Ve or death while other remain +ve or viable. Tolerance level of ureolytic bacteria growth regarding to pH and temperature results were also computed and found that at pH3-6 none are viable, but at pH7 there was an appreciable and significant growth. Similarly to the temperature at 24OC there was no trace of microbial growth, at 27OC-33OC there was an appreciable and maximum growth with immediate alacrity. Nutritional values of the carrier base or organic waste like those of bean pod waste, rice bran waste, banana peel waste etc., respectively,(agro- waste  bio stimulants or carrier  base ) were observed and there were significant  increase  of nutritional values for both plant and microorganisms growth without qualm. Proximate analysis of the nutritional values of both organic base urease bio-fertilizer/ bio-seed bio-fertilizer like those of Ash, moisture crude proteins etc., respectively were cautiously accentuated in situ. The study regarding to the results or findings were checkmated to have mostly found that the organic bio-fertilizer are far much higher than the urea chemical recipes.  Further  investigations  regarding  to the study suggest that ureolytic strain of bacteria isolated might  be  a  potential candidate  for application   through  a sustainable agriculture practices  in developing countries.  In  view  of the  current  study, urea   must  have  had  been  known  as a  major  organic  waste  product of protein digestion in  most  vertebrates  and  is  excreted  in urine or found  in  tannery sludge . Some  particular  microorganisms which  might  have had confirmed ability  to  produce  the enzyme  urease. For   lo  the  microbial  ureolytic  urease  is basically    known  to  be  a hydrolytic  enzyme;  which attacks the carbon and  nitrogen  bond amide compound e.g. urea and  thereby  liberate ammonia as  a potential candidate for  urease  bio-fertilizer which help in soil fertilization without applying chemical fertilizers.

Key words: Ureolytic strains of bacteria,  tannery  sludge,  vertebrate excrete  , urease,  Bio-fertilizer production, Multiple carrier organic base waste, Macro element/ microelement